Running individual WGCNA analyses on male and female sets of oysters. Initial code can be found here. General notes about first run:

  • Using signed networks (those that take into account the direction of expression as well as the value of the count - up or down)
  • Blockwise clustering for module formation, usually good for large datasets, probably not necessary since there isn’t too much data
  • Current status: Switched from this analysis at module merging stage since none of the modules merged with a stringent similarity setting

Male modules and clustering:

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Female modules and clustering:

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Attempt at merging, showing males only in this case. Module similarity did not meet threshold to merge any of the many modules:

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Paused this to attempt Ariana’s methodology which has more stringent filtering steps.