I ran HISAT2 to get alignments for the E5 Porites data using P. lobata and P. evermanni references from here. Looks like the P. evermanni reference wins out with higher overall alignments.
Porites lobata
| Run | Paired Reads | Aligned Concordantly 0 Times | Aligned Concordantly Exactly 1 Time | Aligned Concordantly >1 Times | Aligned Discordantly 1 Time | Overall Alignment Rate |
|---|---|---|---|---|---|---|
| 1 | 50,831,351 | 16,973,703 | 8,204,327 | 25,653,321 | 510,835 | 73.58% |
| 2 | 51,385,213 | 12,840,566 | 5,019,290 | 33,525,357 | 327,247 | 79.12% |
| 3 | 49,828,147 | 15,597,984 | 7,821,811 | 26,408,352 | 440,009 | 74.77% |
| 4 | 49,976,568 | 10,802,392 | 5,426,523 | 33,747,653 | 349,808 | 82.63% |
| 5 | 48,908,730 | 20,325,695 | 9,895,038 | 18,687,997 | 609,205 | 67.15% |
Porites evermanni
| Run | Paired Reads | Aligned Concordantly 0 Times | Aligned Concordantly Exactly 1 Time | Aligned Concordantly >1 Times | Aligned Discordantly 1 Time | Overall Alignment Rate |
|---|---|---|---|---|---|---|
| 1 | 50,831,351 | 11,712,763 | 30,209,320 | 8,909,268 | 2,145,413 | 86.40% |
| 2 | 51,385,213 | 11,914,068 | 28,680,866 | 10,790,279 | 2,306,668 | 87.10% |
| 3 | 49,828,147 | 11,096,891 | 29,566,240 | 9,165,016 | 1,929,423 | 85.96% |
| 4 | 49,976,568 | 10,514,435 | 27,932,980 | 11,529,153 | 2,532,283 | 89.07% |
| 5 | 48,908,730 | 12,403,366 | 28,498,490 | 8,006,874 | 2,215,956 | 84.18% |